8GV5

Crystal structure of PN-SIA28 in complex with influenza hemagglutinin A/swine/Guangdong/104/2013 (H1N1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97774
Spacegroup name	P 63
Unit cell lengths	85.797, 85.797, 231.289
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.630 - 3.200
R-factor	0.2697
Rwork	0.267
R-free	0.30820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5gjs
RMSD bond length	0.005
RMSD bond angle	0.966
Data reduction software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.310
High resolution limit [Å]	3.200	3.200
Rmerge	0.190	2.400
Number of reflections	15923	1571
<I/σ(I)>	15.3	1
Completeness [%]	73.2
Redundancy	12.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1M magnesium acetate, 0.1M sodium acetate, 8% w/v PEG 8000 (pH 4.5)