8GUH

Serine Palmitoyltransferase from Sphingobacterium multivorum complexed with Tris

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-X
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2019-09-09
Detector	RIGAKU RAXIS VII
Wavelength(s)	1.5418
Spacegroup name	P 41 21 2
Unit cell lengths	61.321, 61.321, 208.573
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.031 - 1.650
Rwork	0.170
R-free	0.21430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3a2b
RMSD bond length	0.011
RMSD bond angle	1.675
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.750
High resolution limit [Å]	1.650	1.650
Rmeas	0.063	0.222
Rpim	0.024	0.128
Number of reflections	47616	6313
<I/σ(I)>	23.4	6.4
Completeness [%]	96.8
Redundancy	8.9
CC(1/2)	0.999	0.956

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	PEG4000, sodium acetate, Tris-HCl