8GUG
Structure of VPA0770 toxin bound to VPA0769 antitoxin in Vibrio parahaemolyticus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-09 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97852 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 81.915, 104.725, 116.218 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.480 - 2.850 |
| R-factor | 0.2487 |
| Rwork | 0.247 |
| R-free | 0.27440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Predicted model |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.519 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.333 | 2.930 |
| High resolution limit [Å] | 2.840 | 2.840 |
| Rmeas | 0.142 | 0.722 |
| Number of reflections | 11673 | 861 |
| <I/σ(I)> | 15.8 | |
| Completeness [%] | 96.8 | 83.2 |
| Redundancy | 7.4 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.2 M Calcium chloride dihydrate, 20% w/v Polyethylene glycol 3,350 |
