8GU3

Crystal structure of Caenorhabditis elegans METT-10 methyltransferase domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-02
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	43.210, 70.080, 94.230
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.120 - 3.010
R-factor	0.2438
Rwork	0.241
R-free	0.29750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6b91
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.120	47.120	3.080
High resolution limit [Å]	3.010	13.440	3.010
Rmerge	0.113	0.034	1.341
Rmeas	0.117	0.036	1.398
Total number of observations	76348
Number of reflections	6086	82	448
<I/σ(I)>	17.92	44.17	1.59
Completeness [%]	99.9	96.5	99.1
Redundancy	12.545	8.939	12.377
CC(1/2)	0.999	0.999	0.691

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	ammonium acetate, Tris-HCl pH8.5, PEG 3350