8GTY
Crystal structure of exopolyphosphatase (PPX) from Zymomonas mobilis in complex with magnesium ions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 122.023, 47.118, 89.480 |
| Unit cell angles | 90.00, 124.45, 90.00 |
Refinement procedure
| Resolution | 41.510 - 1.800 |
| Rwork | 0.200 |
| R-free | 0.24430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hi0 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | BALBES |
| Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.840 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.119 | 0.515 |
| Rmeas | 0.136 | 0.620 |
| Rpim | 0.065 | 0.336 |
| Number of reflections | 36563 | 2697 |
| <I/σ(I)> | 8.7 | |
| Completeness [%] | 92.7 | 67.4 |
| Redundancy | 4 | 3.1 |
| CC(1/2) | 0.645 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 293 | 50 mM Tris pH 8.0, 500 mM NaCl, 5% glycerol 0.2 M magnesium chloride hexahydrate, 0.1 M Tris pH 8.5, 25%(w/v) polyethylene glycol 3350 |
