8GSY

X-ray structure of Clostridium perfringens pili protein B N-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-22
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	P 32 2 1
Unit cell lengths	39.080, 39.080, 290.590
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.480 - 1.550
R-factor	0.1868
Rwork	0.184
R-free	0.23963
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8gsx
RMSD bond length	0.008
RMSD bond angle	1.502
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.480	1.590
High resolution limit [Å]	1.550	1.550
Number of reflections	39168	2809
<I/σ(I)>	23.23
Completeness [%]	100.0
Redundancy	9.4
CC(1/2)	0.999	0.789

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	50 mM bis-tris pH 6.5, 50 mM ammonium sulfate, 30% v/v pentaerythritol ethoxylate (15/4 EO/OH)