8GRW

Spiroplasma melliferum FtsZ F224M bound to GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 11.2C
Synchrotron site	ELETTRA
Beamline	11.2C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 41 21 2
Unit cell lengths	106.669, 106.669, 128.781
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.276 - 2.400
R-factor	0.195785312543
Rwork	0.193
R-free	0.23771
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ysz
RMSD bond length	0.007
RMSD bond angle	0.832
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	106.670	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.096	0.684
Number of reflections	29504	3038
<I/σ(I)>	15.1
Completeness [%]	99.5
Redundancy	7
CC(1/2)	0.998	0.785

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291.15	0.3M sodium acetate, 0.1M Tris pH 8.5, 15% w/v PEG 4000