8GR7
Cop4 from Antrodia cinnamomea in complex with pyrophosphate and magnesium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2021-04-09 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 45.203, 45.224, 79.324 |
| Unit cell angles | 85.06, 78.05, 79.95 |
Refinement procedure
| Resolution | 27.980 - 1.900 |
| R-factor | 0.1644 |
| Rwork | 0.162 |
| R-free | 0.20610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8gr5 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rpim | 0.040 | |
| Number of reflections | 46461 | 4628 |
| <I/σ(I)> | 17.8 | |
| Completeness [%] | 97.6 | |
| Redundancy | 4 | |
| CC(1/2) | 0.891 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 283 | HEPES pH 7.5, 10% 2-Propanol, 20% PEG 4000 |
