8GQV

The Crystal Structures of a Swine SLA-2*HB01 Molecules Complexed with a CTL epitope from Asia1 serotype of Foot-and-mouth disease virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-10-08
Detector	MAATEL IMAGINE
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	48.465, 98.143, 166.006
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.202 - 2.400
R-factor	0.2069
Rwork	0.204
R-free	0.24900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1q94
RMSD bond length	0.005
RMSD bond angle	1.003
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.078	0.318
Number of reflections	31827	31827
<I/σ(I)>	21.8
Completeness [%]	99.2
Redundancy	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	Ammonium acetate, PEG, BIS-TRIS