8GQ4

Histone acetyltransferase Rtt109 mutant-N195A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	126.15
Detector technology	PIXEL
Collection date	2017-08-03
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9754
Spacegroup name	P 1 21 1
Unit cell lengths	51.768, 69.840, 54.585
Unit cell angles	90.00, 114.22, 90.00

Refinement procedure

Resolution	49.780 - 1.770
R-factor	0.1908
Rwork	0.188
R-free	0.23410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7bxw
RMSD bond length	0.012
RMSD bond angle	2.012
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.820
High resolution limit [Å]	1.770	1.770
Rmerge	0.185	0.335
Number of reflections	34445	2832
<I/σ(I)>	11.42
Completeness [%]	100.0
Redundancy	7.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291.15	0.1 M Bis-Tris pH 6.0, 2% Tacsimate pH 6.0, 15~20% PEG 3350