8GPV

Cytoplasmic domain structure of the MgtE Mg2+ channel from Clostridiales bacterium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97918
Spacegroup name	P 43 2 2
Unit cell lengths	81.063, 81.063, 104.565
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	81.060 - 1.820
R-factor	0.2263
Rwork	0.225
R-free	0.25740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2yvy
RMSD bond length	0.008
RMSD bond angle	1.063
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	81.060	1.910
High resolution limit [Å]	1.820	1.820
Number of reflections	28748	4197
<I/σ(I)>	38.1
Completeness [%]	89.3
Redundancy	25
CC(1/2)	1.000	0.877

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	293	0.2 M sodium chloride, 0.1 M MOPS pH 7.5, 38% v/v PEG 400