8GO7

Fungal immunomodulatory protein FIP-nha N5+39A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	118.311, 101.304, 74.877
Unit cell angles	90.00, 129.19, 90.00

Refinement procedure

Resolution	28.981 - 2.303
R-factor	0.2254
Rwork	0.223
R-free	0.26030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7wdl
Data reduction software	HKL-2000
Data scaling software	HKL-3000 (7.21)
Phasing software	PHASER (2.7.0)
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.340
High resolution limit [Å]	2.300	6.240	2.300
Rmerge	0.123	0.117	0.441
Rmeas	0.136	0.128	0.481
Rpim	0.055	0.051	0.190
Total number of observations	182087
Number of reflections	29149	1526	1524
<I/σ(I)>	9.2
Completeness [%]	96.3	98.3	100
Redundancy	6.2	6.2	6.3
CC(1/2)		0.988	0.916

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1M Sodium choride, 0.1M Bis-Tris pH 6.5, 1.5M Ammonium Sulfate