8GO6

Fungal immunomodulatory protein FIP-nha N39A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	135.251, 75.284, 89.615
Unit cell angles	90.00, 131.55, 90.00

Refinement procedure

Resolution	44.807 - 2.813
R-factor	0.1998
Rwork	0.197
R-free	0.25060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7wdl
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.900
High resolution limit [Å]	2.800	6.030	2.800
Rmerge	0.140	0.064	0.714
Rmeas	0.153	0.071	0.773
Rpim	0.061	0.029	0.294
Total number of observations	97659
Number of reflections	15619	1574	1625
<I/σ(I)>	5.6
Completeness [%]	95.3	93	99.9
Redundancy	6.3	5.7	6.8
CC(1/2)		0.995	0.805

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1M sodium chloride, 0.1M Bis-tris pH 6.5, 1.5M Ammonium Sulfate