8GN3

The crystal structure of ZBTB10 ZF1-2 in complex with telomeric vairant repeat TTGGGG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	36.119, 82.304, 82.389
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.080 - 1.800
R-factor	0.2013
Rwork	0.200
R-free	0.22500
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	1.830
High resolution limit [Å]	1.800	4.880	1.800
Rmerge	0.061	0.023	1.145
Rmeas	0.063	0.024	1.204
Rpim	0.018	0.007	0.362
Total number of observations	297558
Number of reflections	23647	1318	1144
<I/σ(I)>	6.9
Completeness [%]	99.9	99.8	99.4
Redundancy	12.6	12	10.3
CC(1/2)		1.000	0.700

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Sodium HEPES, pH 8.2, 40 % v/v PEG 500 MME