8GN1
Crystal structure of DBBQ-bound photosystem II complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2021-04-13 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 0.9 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 122.185, 228.462, 286.626 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.020 - 2.100 |
R-factor | 0.159 |
Rwork | 0.157 |
R-free | 0.19120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3wu2 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.235 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.072 | 1.471 |
Number of reflections | 460342 | 45180 |
<I/σ(I)> | 14.2 | 1.3 |
Completeness [%] | 99.2 | |
Redundancy | 5.4 | |
CC(1/2) | 0.999 | 0.653 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 6.1 | 285 | 5% PEG1450, 20mM NaCl, 10mM CaCl2, 40mM MgSO4, 20mM MES buffer, pH 6.1, MICRO-BATCH METHOD UNDER OIL, temperature 285K |