8GMY

Structure of methyltransferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-21
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 65
Unit cell lengths	98.436, 98.436, 41.628
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.220 - 1.700
R-factor	0.2164
Rwork	0.215
R-free	0.24290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	alphafold2
RMSD bond length	0.007
RMSD bond angle	0.912
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.220	1.800
High resolution limit [Å]	1.700	1.700
Number of reflections	25095	3392
<I/σ(I)>	22.6
Completeness [%]	98.8
Redundancy	11.7
CC(1/2)	1.000	0.846

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291.15	PEG550 MME, MES/Sodiumhydroxid