8GMW

Crystal structure of lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2022-07-19
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97933
Spacegroup name	P 43 21 2
Unit cell lengths	78.941, 78.941, 37.106
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.150 - 1.350
R-factor	0.1801
Rwork	0.179
R-free	0.19420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7wuc
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.370
High resolution limit [Å]	1.350	1.350
Number of reflections	25439	1164
<I/σ(I)>	30.59
Completeness [%]	96.6
Redundancy	9.4
CC(1/2)	0.995	0.647

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	4.6	298	Na-acetate, NaCl, PEG 8000