8GM7

Structure of apurinic/apyrimidinic DNA Lyase TK0353 from Thermococcus kodakarensis (Native Crystal Form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.033
Spacegroup name	P 21 21 21
Unit cell lengths	62.212, 89.925, 130.922
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.650 - 1.750
R-factor	0.1908
Rwork	0.188
R-free	0.22020
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.208
Data reduction software	PROTEUM PLUS (3)
Data scaling software	PROTEUM PLUS (3)
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.000	1.810
High resolution limit [Å]	1.750	1.750
Rmerge	0.109	2.035
Rmeas	0.121	2.268
Rpim	0.051	0.987
Number of reflections	139905	13696
<I/σ(I)>	6.6	0.9
Completeness [%]	99.2	92.6
Redundancy	5.4	5.2
CC(1/2)	0.994	0.541

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		297	2.5 M (NH4)2SO4, cryoprotection by addition of 1.5M Li2SO4