8GKV
Crystal structure of anti-adaptor IraP that regulates RpoS proteolysis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-22 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 75.335, 57.013, 48.629 |
| Unit cell angles | 90.00, 129.50, 90.00 |
Refinement procedure
| Resolution | 29.064 - 2.351 |
| R-factor | 0.2238 |
| Rwork | 0.215 |
| R-free | 0.23860 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.745 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.430 |
| High resolution limit [Å] | 2.350 | 5.060 | 2.350 |
| Rmerge | 0.051 | 0.035 | 0.156 |
| Total number of observations | 21575 | ||
| Number of reflections | 6391 | 686 | 514 |
| <I/σ(I)> | 16 | ||
| Completeness [%] | 95.4 | 98.4 | 77.4 |
| Redundancy | 3.4 | 3.5 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 292 | Tri-Na citrate, MPD |
