8GKV

Crystal structure of anti-adaptor IraP that regulates RpoS proteolysis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-22
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	75.335, 57.013, 48.629
Unit cell angles	90.00, 129.50, 90.00

Refinement procedure

Resolution	29.064 - 2.351
R-factor	0.2238
Rwork	0.215
R-free	0.23860
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	0.745
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.430
High resolution limit [Å]	2.350	5.060	2.350
Rmerge	0.051	0.035	0.156
Total number of observations	21575
Number of reflections	6391	686	514
<I/σ(I)>	16
Completeness [%]	95.4	98.4	77.4
Redundancy	3.4	3.5	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	292	Tri-Na citrate, MPD