8GKL

Crystal Structure of the Humanized MUC16 Specific Antibody huAR9.6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-01-26
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.521
Spacegroup name	C 2 2 21
Unit cell lengths	41.470, 124.360, 235.430
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.740 - 2.600
R-factor	0.2181
Rwork	0.215
R-free	0.26920
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.474
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.740	2.693
High resolution limit [Å]	2.600	2.600
Rpim	0.064	0.376
Number of reflections	212625	17395
<I/σ(I)>	8.6
Completeness [%]	99.2
Redundancy	11.1
CC(1/2)	0.959	0.785

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M PCTP pH 4.0 25% PEG 1500