8GJY
Structure of a cGAS-like receptor Sp-cGLR1 from S. pistillata
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2022-11-17 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 165.160, 41.319, 58.649 |
| Unit cell angles | 90.00, 91.80, 90.00 |
Refinement procedure
| Resolution | 48.520 - 1.500 |
| R-factor | 0.1709 |
| Rwork | 0.171 |
| R-free | 0.18010 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.816 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.520 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 63374 | 6220 |
| <I/σ(I)> | 15.6 | |
| Completeness [%] | 99.5 | 98.3 |
| Redundancy | 3.5 | 3.4 |
| CC(1/2) | 0.997 | 0.949 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 275 mM Ammonium nitrate, 16% (w/v) PEG3350 |
