8GJ6
LSD1-CoREST in complex with T16
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-02 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.965459 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 118.355, 178.664, 234.966 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.460 - 2.770 |
| R-factor | 0.224 |
| Rwork | 0.223 |
| R-free | 0.25120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.414 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.460 | 2.840 |
| High resolution limit [Å] | 2.770 | 2.770 |
| Rmerge | 0.106 | 1.670 |
| Rmeas | 0.125 | 1.967 |
| Rpim | 0.065 | 1.021 |
| Total number of observations | 208431 | 15792 |
| Number of reflections | 61770 | 4585 |
| <I/σ(I)> | 7.2 | 0.8 |
| Completeness [%] | 97.5 | |
| Redundancy | 3.4 | 3.4 |
| CC(1/2) | 0.991 | 0.275 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 1.2 Sodium/Potassium Tartrate, 100 mM ADA pH 6.5 |
