8GIL
L-threonine 3-Dehydrogenase from Trypanosoma cruzi (apo form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS SIRIUS BEAMLINE MANACA |
| Synchrotron site | LNLS SIRIUS |
| Beamline | MANACA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-04-02 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.977180 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.685, 81.834, 83.674 |
| Unit cell angles | 90.00, 90.55, 90.00 |
Refinement procedure
| Resolution | 46.727 - 2.100 |
| Rwork | 0.200 |
| R-free | 0.25810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.744 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.730 | 2.160 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 36424 | 2927 |
| <I/σ(I)> | 8.4 | |
| Completeness [%] | 99.1 | 98.6 |
| Redundancy | 4.7 | 4.5 |
| CC(1/2) | 0.995 | 0.595 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291.15 | 25% PEG 3350, 200 mM ammonium acetate and 100 mM Bis-Tris pH 5.5 |
