8GI3
Crystal structure of RhoA mutant L69P complexed with GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2022-06-16 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 32.048, 64.081, 82.504 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.660 - 1.520 |
| R-factor | 0.1725 |
| Rwork | 0.171 |
| R-free | 0.20947 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.896 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | MOLREP (v11.0) |
| Refinement software | REFMAC (v5.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.660 | 1.550 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Rmerge | 0.110 | 0.143 |
| Rmeas | 0.120 | 0.154 |
| Rpim | 0.046 | 0.058 |
| Number of reflections | 26852 | 2615 |
| <I/σ(I)> | 13.1 | 10.6 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 6.5 | 6.6 |
| CC(1/2) | 0.988 | 0.980 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 5.1 | 293.2 | 0.2 M Ammonium citrate dibasic (pH 5.1), 20% PEG3350 |
