8GHI
Crystal structure of Staphylococcus aureus Lysophosphatidylglycerol phospholipase D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-10-22 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 108.032, 108.032, 139.710 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.200 - 2.400 |
| R-factor | 0.2258 |
| Rwork | 0.225 |
| R-free | 0.24940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.468 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.660 | 2.450 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.119 | 1.041 |
| Number of reflections | 15817 | 1014 |
| <I/σ(I)> | 7.4 | 1.1 |
| Completeness [%] | 95.7 | 99.7 |
| Redundancy | 4.7 | 5.4 |
| CC(1/2) | 0.989 | 0.447 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | Phosphate-citrate, sodium dihydrogen phosphate, dipotassium hydrogen phosphate |
