8GF4
Crystal structure of Domain Related to Iron (DRI) in complex with heme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-2 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-09-15 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979 |
Spacegroup name | P 61 |
Unit cell lengths | 42.976, 42.976, 379.484 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.250 - 3.000 |
R-factor | 0.20602 |
Rwork | 0.201 |
R-free | 0.27914 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 2.080 |
Data reduction software | autoPROC |
Data scaling software | autoPROC |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.080 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.092 | 0.504 |
Number of reflections | 7898 | 830 |
<I/σ(I)> | 6 | |
Completeness [%] | 92.6 | |
Redundancy | 3.5 | |
CC(1/2) | 0.980 | 0.969 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 23% PEG3350, 100 mM Bis-Tris, pH 5.5 |