8GDX
Crystal structure of JADE1 PZP domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-22 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 81.439, 81.439, 81.439 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.790 - 2.740 |
| R-factor | 0.2222 |
| Rwork | 0.220 |
| R-free | 0.26870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5erc |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.790 | 2.790 |
| High resolution limit [Å] | 2.740 | 2.740 |
| Rpim | 0.082 | 0.298 |
| Number of reflections | 4947 | 230 |
| <I/σ(I)> | 8.5 | |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 5.2 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 2 M Ammonium sulfate, 0.1 M Sodium acetate, pH 4.6 |
