8GDW
Crystal structure of Domain Related to Iron (DRI) from cyanobacteria
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-2 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-09-15 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.716, 87.230, 57.241 |
| Unit cell angles | 90.00, 94.98, 90.00 |
Refinement procedure
| Resolution | 29.500 - 2.350 |
| Rwork | 0.197 |
| R-free | 0.24740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5bnc |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.545 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.500 | 2.410 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.055 | 0.736 |
| Number of reflections | 20972 | 1362 |
| <I/σ(I)> | 15 | 1.9 |
| Completeness [%] | 99.0 | 87.8 |
| Redundancy | 5.3 | 5 |
| CC(1/2) | 0.990 | 0.760 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 25% PEG3350, 100 mM Bis-Tris, pH 5.5 |
