8GCJ
PCNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-06-22 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 147.104, 85.186, 149.806 |
| Unit cell angles | 90.00, 116.87, 90.00 |
Refinement procedure
| Resolution | 48.390 - 2.850 |
| R-factor | 0.2336 |
| Rwork | 0.231 |
| R-free | 0.27750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.111 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.390 | 2.980 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.058 | 0.300 |
| Rmeas | 0.063 | 0.324 |
| Rpim | 0.024 | 0.121 |
| Total number of observations | 261519 | 32613 |
| Number of reflections | 38188 | 4618 |
| <I/σ(I)> | 17.5 | 5.7 |
| Completeness [%] | 98.5 | |
| Redundancy | 6.8 | 7.1 |
| CC(1/2) | 0.999 | 0.982 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289.15 | 0.2 M Magnesium chloride hexahydrate, 0.1 M HEPES pH 7.5, 25% w/v Polyethylene glycol 3,350 |
