8GCJ
PCNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-06-22 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9537 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 147.104, 85.186, 149.806 |
Unit cell angles | 90.00, 116.87, 90.00 |
Refinement procedure
Resolution | 48.390 - 2.850 |
R-factor | 0.2336 |
Rwork | 0.231 |
R-free | 0.27750 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.111 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHENIX |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.390 | 2.980 |
High resolution limit [Å] | 2.850 | 2.850 |
Rmerge | 0.058 | 0.300 |
Rmeas | 0.063 | 0.324 |
Rpim | 0.024 | 0.121 |
Total number of observations | 261519 | 32613 |
Number of reflections | 38188 | 4618 |
<I/σ(I)> | 17.5 | 5.7 |
Completeness [%] | 98.5 | |
Redundancy | 6.8 | 7.1 |
CC(1/2) | 0.999 | 0.982 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289.15 | 0.2 M Magnesium chloride hexahydrate, 0.1 M HEPES pH 7.5, 25% w/v Polyethylene glycol 3,350 |