Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-09-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97741 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.269, 44.360, 58.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.290 - 1.470 |
| R-factor | 0.206 |
| Rwork | 0.205 |
| R-free | 0.23350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.572 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.360 | 1.500 |
| High resolution limit [Å] | 1.470 | 1.470 |
| Rmerge | 0.050 | 0.804 |
| Number of reflections | 18834 | 911 |
| <I/σ(I)> | 24.5 | 3 |
| Completeness [%] | 100.0 | 99.5 |
| Redundancy | 12.4 | 12.7 |
| CC(1/2) | 0.990 | 0.850 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 2.0 M Ammonium sulfate 0.2M Sodium/Potassium Tartrate |
