8GBZ
Crystal structure of PC39-55C, an anti-HIV broadly neutralizing antibody
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-04-18 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97946 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 39.366, 171.380, 68.415 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.850 - 1.970 |
R-factor | 0.2211 |
Rwork | 0.219 |
R-free | 0.26480 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.744 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.000 |
High resolution limit [Å] | 1.970 | 1.970 |
Rpim | 0.030 | 0.250 |
Number of reflections | 33396 | 1605 |
<I/σ(I)> | 17.2 | 2.8 |
Completeness [%] | 99.5 | 99.6 |
Redundancy | 7.1 | 6 |
CC(1/2) | 0.980 | 0.890 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.2 M ammonium fluoride, 15% ethylene glycol, 20% PEG 3350 |