8GAI

Cavia porcellus (guinea pig) importin-alpha 1 in complex with Bimax2 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	75.008, 99.269, 123.759
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.950 - 2.650
R-factor	0.2044
Rwork	0.202
R-free	0.24110
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.668
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.950	2.780
High resolution limit [Å]	2.650	2.650
Number of reflections	25741	24373
<I/σ(I)>	6.5
Completeness [%]	94.5
Redundancy	4.5
CC(1/2)	0.991	0.689

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	296	PEG6000, 0.1M sodium acetate, 0.2M ammonium chloride