8GAC
Crystal structure of a high affinity CTLA-4 binder
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-09-22 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 39.865, 52.037, 53.524 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.850 - 1.850 |
Rwork | 0.191 |
R-free | 0.21860 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | autoPROC |
Data scaling software | Aimless |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.850 | 1.920 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.080 | |
Number of reflections | 9931 | 1170 |
<I/σ(I)> | 18.4 | |
Completeness [%] | 99.6 | |
Redundancy | 10.6 | |
CC(1/2) | 0.990 | 0.430 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 292 | 30 % (w/v) PEG 3350, 0.22 M MgCl2 and 0.0.8 M Bis-Tris pH 5.5 and 0.15 M HEPES |