8GA7
Geometrically programmable nanomaterial construction using regularized protein building blocks
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-08-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.99999 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.412, 65.062, 135.284 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.270 - 2.930 |
| R-factor | 0.2792 |
| Rwork | 0.275 |
| R-free | 0.31470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.517 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_4761) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.270 | 3.080 |
| High resolution limit [Å] | 2.930 | 2.930 |
| Rmerge | 0.204 | 1.178 |
| Rpim | 0.079 | 0.439 |
| Number of reflections | 9149 | 1290 |
| <I/σ(I)> | 7.79 | |
| Completeness [%] | 98.3 | 98.93 |
| Redundancy | 7.5 | |
| CC(1/2) | 0.997 | 0.939 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.2 M Ammonium acetate, 0.1 M HEPES pH 7.5, 25% w/v Polyethylene glycol 3,350 |
