8G9K

Geometrically programmable nanomaterial construction using regularized protein building blocks

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-08
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97648
Spacegroup name	P 21 21 21
Unit cell lengths	46.394, 61.195, 149.025
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.610 - 2.480
R-factor	0.266
Rwork	0.262
R-free	0.29940
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.001
RMSD bond angle	0.376
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18rc2_3793)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.610	2.560
High resolution limit [Å]	2.480	2.480
Rmerge	0.063	0.123
Rpim	0.025	0.491
Number of reflections	15526	1388
<I/σ(I)>	17.19
Completeness [%]	98.9	98.79
Redundancy	7.4	7.6
CC(1/2)	0.999	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.3M Sodium nitrate, 0.3M Sodium phosphate dibasic, 0.3M Ammonium sulfate, 0.1M Sodium HEPES; MOPS(acid) pH 7.5, 30% mixture of 40% v/v Ethylene glycol; 20% w/v PEG 8000