8G9J
Geometrically programmable nanomaterial construction using regularized protein building blocks
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2020-03-10 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.99993 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 49.701, 49.701, 129.260 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.390 - 2.500 |
R-factor | 0.2386 |
Rwork | 0.233 |
R-free | 0.28490 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.517 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (dev_4761) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.390 | 2.750 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.069 | 0.431 |
Rpim | 0.069 | |
Number of reflections | 6005 | 1404 |
<I/σ(I)> | 6.62 | 1.2 |
Completeness [%] | 98.6 | 95.58 |
Redundancy | 1.7 | 1.3 |
CC(1/2) | 0.999 | 0.870 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.2 M Magnesium chloride hexahydrate, 0.1 M BIS-TRIS pH 5.5, and 25% w/v Polyethylene glycol 3,350 |