8G91

LaM domain of human LARP1 in complex with Rp phosphorothioate isomer of AAAAA(SRA) RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-29
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.95371
Spacegroup name	P 43 21 2
Unit cell lengths	53.781, 53.781, 90.452
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.060 - 1.200
R-factor	0.1788
Rwork	0.178
R-free	0.18930
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.171
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.220
High resolution limit [Å]	1.200	1.200
Number of reflections	41809	3876
<I/σ(I)>	42.4	1.2
Completeness [%]	99.4	96.6
Redundancy	20	10.3
CC(1/2)	0.999	0.592

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	0.1 M ammonium acetate, 0.1 M Bis-Tris pH 5.5, 17% PEG 10000