8G90
LaM domain of human LARP1 in complex with Sp phosphorothioate isomer of AAAAA(SRA) RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-07-27 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.95371 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.951, 46.646, 59.276 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.020 - 1.200 |
| R-factor | 0.1928 |
| Rwork | 0.192 |
| R-free | 0.20960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.937 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.220 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Number of reflections | 31018 | 2236 |
| <I/σ(I)> | 35.6 | 2.8 |
| Completeness [%] | 95.2 | 67.7 |
| Redundancy | 8.6 | 2.9 |
| CC(1/2) | 0.993 | 0.825 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 0.1 M Bis-Tris pH 6.5, 28% (w/v) PEG 2000 MME |
