8G8R

RelB NLS in complex with Importin alpha 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	78.465, 90.328, 99.501
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.840 - 2.600
R-factor	0.1794
Rwork	0.178
R-free	0.21280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6bw0
RMSD bond length	0.003
RMSD bond angle	0.605
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.840	2.600
High resolution limit [Å]	2.600	2.600
Number of reflections	22000	1053
<I/σ(I)>	6.9
Completeness [%]	88.7
Redundancy	6.1
CC(1/2)	0.989	0.841

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	0.01M DTT, 0.1M sodium HEPES pH7.5, 0.8.5M sodium citrate