8G8N
CTLA4 Fab with peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2018-03-14 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.430, 203.510, 225.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.030 - 3.000 |
| R-factor | 0.2414 |
| Rwork | 0.239 |
| R-free | 0.28820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.542 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.030 | 3.107 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.270 | 0.822 |
| Rmeas | 0.322 | |
| Rpim | 0.173 | |
| Number of reflections | 66307 | 6562 |
| <I/σ(I)> | 4.08 | |
| Completeness [%] | 97.8 | |
| Redundancy | 3.3 | |
| CC(1/2) | 0.963 | 0.534 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2 M Ammonium Citrate Dibasic pH 5.0 with 20% PEG 3350 |
