8G6D

HSV-1 Nuclear Egress Complex (SUP; UL31-R229L)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9786
Spacegroup name	C 1 2 1
Unit cell lengths	109.555, 189.103, 157.096
Unit cell angles	90.00, 100.52, 90.00

Refinement procedure

Resolution	94.550 - 3.920
R-factor	0.2578
Rwork	0.255
R-free	0.30110
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.695
Data reduction software	xia2 (0.6.467)
Data scaling software	xia2 (0.6.467)
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	94.550	4.060
High resolution limit [Å]	3.920	3.920
Number of reflections	28047	2801
<I/σ(I)>	2.83
Completeness [%]	99.1	98.83
Redundancy	6.7	6.5
CC(1/2)	0.591	0.312

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	10% PEG 3350, 8 mM lithium sulfate, 6 mM ATP, 0.1 M MES, and Silver Bullets reagent G3