Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2021-10-20 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 117.522, 117.522, 72.073 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.570 - 2.820 |
| R-factor | 0.2157 |
| Rwork | 0.214 |
| R-free | 0.24920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.452 |
| Data reduction software | HKL-2000 (v721.3) |
| Data scaling software | HKL-2000 (v721.3) |
| Phasing software | PHENIX (1.20.1_4487) |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.570 | 2.921 |
| High resolution limit [Å] | 2.820 | 2.820 |
| Rmerge | 0.269 | 1.397 |
| Rmeas | 0.276 | 1.453 |
| Rpim | 0.060 | 0.395 |
| Number of reflections | 14120 | 1357 |
| <I/σ(I)> | 16.02 | 1.73 |
| Completeness [%] | 99.1 | 97.07 |
| Redundancy | 20.6 | 13 |
| CC(1/2) | 0.984 | 0.672 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M NaCl, 0.1 M BIS TRIS, 1.3 - 1.7 M ammonium sulfate |
