8G6B
Crystal structure of PfAMA1-RON2L chimera
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-01-30 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.984 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 92.499, 92.499, 173.681 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 57.894 - 1.550 |
| R-factor | 0.1826 |
| Rwork | 0.181 |
| R-free | 0.21120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.139 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.880 | 1.580 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.075 | 0.624 |
| Number of reflections | 124774 | 6114 |
| <I/σ(I)> | 11.4 | |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 0.1 M Tris-HCl pH 7.0, 1.5 M lithium sulfate |
