8G5S

Crystal structure of apo TnmJ

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-09
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	58.784, 100.908, 58.836
Unit cell angles	90.00, 102.86, 90.00

Refinement procedure

Resolution	29.020 - 1.500
R-factor	0.1678
Rwork	0.166
R-free	0.19520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5f8c
RMSD bond length	0.009
RMSD bond angle	1.009
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.020	1.556
High resolution limit [Å]	1.500	1.500
Rmerge	0.057	0.090
Number of reflections	98178	7779
<I/σ(I)>	9.96
Completeness [%]	93.5
Redundancy	1.54
CC(1/2)	0.986	0.927

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.2 M magnesium chloride, 0.1 M Tris-HCl pH 8.5, and 25% (v/v) PEG 3350