8G4J

Crystal structure of Cavia porcellus (guinea pig) importin-alpha 1 in cargo-free state

Replaces:  7S1E

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 4 21 2
Unit cell lengths	184.169, 184.169, 57.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.980 - 2.350
R-factor	0.216
Rwork	0.215
R-free	0.23540
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.569
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.410	2.440
High resolution limit [Å]	2.350	2.350
Rmerge	0.098	1.296
Rpim	0.027	0.370
Number of reflections	41876	41860
<I/σ(I)>	12.1	1.8
Completeness [%]	99.8
Redundancy	14
CC(1/2)	0.999	0.780

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296	0.1 M MgCl2.6H2O; 0.1 M Na+ HEPES; 15%(w/v); pH 7.0; PEG4000