8G2K

Structure of the H3 hemagglutinin of A/California/7/2004

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2010-11-09
Detector	RIGAKU RAXIS
Wavelength(s)	1.0
Spacegroup name	H 3 2
Unit cell lengths	101.199, 101.199, 384.462
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.250 - 2.350
R-factor	0.2106
Rwork	0.209
R-free	0.23530
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.608
Data reduction software	MOSFLM (1.0.7)
Data scaling software	SCALA (2.10)
Phasing software	PHASER (2.5.1)
Refinement software	PHENIX ((dev_3973: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.250	2.480
High resolution limit [Å]	2.350	2.350
Rmerge	0.066	0.712
Rmeas	0.073	0.348
Rpim	0.023	0.794
Number of reflections	31998	9617
<I/σ(I)>	19.94	2.6
Completeness [%]	99.5	99.1
Redundancy	9.8	8.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	310.15	Pentaerythritol propoxylate (5/4 PO/OH), 200 mM KCl and 50 mM HEPES (pH 7.5 adjusted with KOH).