8G2K
Structure of the H3 hemagglutinin of A/California/7/2004
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-11-09 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.0 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 101.199, 101.199, 384.462 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.250 - 2.350 |
| R-factor | 0.1988 |
| Rwork | 0.197 |
| R-free | 0.23130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.681 |
| Data reduction software | MOSFLM (1.0.7) |
| Data scaling software | SCALA (2.10) |
| Phasing software | PHASER (2.5.1) |
| Refinement software | PHENIX (1.20) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.250 | 2.480 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.066 | 0.712 |
| Rmeas | 0.073 | 0.348 |
| Rpim | 0.023 | 0.794 |
| Number of reflections | 31998 | 9617 |
| <I/σ(I)> | 19.94 | 2.6 |
| Completeness [%] | 99.5 | 99.1 |
| Redundancy | 9.8 | 8.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 310.15 | Pentaerythritol propoxylate (5/4 PO/OH), 200 mM KCl and 50 mM HEPES (pH 7.5 adjusted with KOH). |
