8G2I
Crystal Structure of PRMT4 with Compound YD1290
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-09-27 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 86.659, 86.659, 137.066 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.730 - 2.170 |
| R-factor | 0.20177 |
| Rwork | 0.201 |
| R-free | 0.22466 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.006 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.210 |
| High resolution limit [Å] | 2.170 | 2.170 |
| Rmerge | 0.069 | 0.991 |
| Number of reflections | 32051 | 1557 |
| <I/σ(I)> | 7.2 | 2 |
| Completeness [%] | 99.6 | 99.2 |
| Redundancy | 16.4 | 13.2 |
| CC(1/2) | 1.000 | 0.851 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 291 | 0.1 M BICINE pH 9.0, 2.0 M Magnesium chloride hexahydrate |
