8G1M
Lectin domain of SasG (Staphylococcus aureus Surface protein G)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 277 |
| Detector technology | CCD |
| Collection date | 2020-11-07 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97872 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 127.099, 43.500, 39.400 |
| Unit cell angles | 90.00, 90.56, 90.00 |
Refinement procedure
| Resolution | 39.407 - 1.930 |
| R-factor | 0.1534 |
| Rwork | 0.148 |
| R-free | 0.19900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.794 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.14-3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.410 | 1.999 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Number of reflections | 16284 | 1613 |
| <I/σ(I)> | 141.31 | 25 |
| Completeness [%] | 99.0 | 98.59 |
| Redundancy | 11.5 | |
| CC(1/2) | 0.801 | 0.756 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.9 | 298 | 100 mM Tris (pH 7.9), 40 mM Na Formate, 40 mM CaCl2, and 14-20% BCS PEG SMEAR Low (Molecular Dimensions, CalibreScientific) |
