8G0K
Bacteroides multispecies type VI secretion system Ntox15 domain effector and immunity Tde1/Tdi1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2020-02-25 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 109.770, 112.272, 149.057 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.790 - 2.200 |
| R-factor | 0.2095 |
| Rwork | 0.207 |
| R-free | 0.25830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.168 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.220 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.100 | 1.110 |
| Number of reflections | 46798 | 1159 |
| <I/σ(I)> | 20.6 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.8 | 10.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 15% PEG 3350, 200 mM lithium sulfate, 100 mM Bis-Tris pH 5.5 |
