8FZY
Bacteroides spp. Ntox15 domain type VI secretion system effector Tde1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2020-05-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 86.472, 149.934, 176.511 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.640 - 2.900 |
| R-factor | 0.204 |
| Rwork | 0.202 |
| R-free | 0.25820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.789 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.640 | 3.240 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.150 | 1.100 |
| Number of reflections | 51580 | 14277 |
| <I/σ(I)> | 13.9 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.6 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 2 M ammonium sulfate, 200 mM sodium acetate pH 4.5 |
